TY - JOUR
T1 - Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress
AU - Zaynab, Madiha
AU - Xu, Zhao Shi
AU - Yad, Hassan A.
AU - Hussain, Athar
AU - Sharif, Yasir
AU - Al-Yahyai, Rashid
AU - Sadder, Monther
AU - Aloufi, Abeer S.
AU - Li, Shuangfei
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the callose synthase genes is limited. In this study, we analyzed the callose synthase gene family of soybean at the genome-wide scale. We also studied the genes positions, gene structure, evolutionary relations, miRNAs target sites, and expression of CalS genes. Resultantly 24 CalS genes were found in soybean, with diverse chromosomal locations, cis-acting elements, conserved motifs, and gene structures. Further, GmCalS genes were divided into four phylogenetic classes. The evolutionary classification of CalSs was supported by the motif and gene structure analyses. Phytohormones, abiotic stresses, and growth-responsive elements were identified in the promoter of GmCalSs. In addition, the GmCalS genes higher expression in roots, leaves, flowers, and nodules tissues provided their significance in development. Furthermore, the higher expression of GmCalS17 and GmCalS19 genes in response to salt stress indicated their importance against salt stress. These findings will be helpful for further investigation of the CalS genes in other crops.
AB - Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the callose synthase genes is limited. In this study, we analyzed the callose synthase gene family of soybean at the genome-wide scale. We also studied the genes positions, gene structure, evolutionary relations, miRNAs target sites, and expression of CalS genes. Resultantly 24 CalS genes were found in soybean, with diverse chromosomal locations, cis-acting elements, conserved motifs, and gene structures. Further, GmCalS genes were divided into four phylogenetic classes. The evolutionary classification of CalSs was supported by the motif and gene structure analyses. Phytohormones, abiotic stresses, and growth-responsive elements were identified in the promoter of GmCalSs. In addition, the GmCalS genes higher expression in roots, leaves, flowers, and nodules tissues provided their significance in development. Furthermore, the higher expression of GmCalS17 and GmCalS19 genes in response to salt stress indicated their importance against salt stress. These findings will be helpful for further investigation of the CalS genes in other crops.
KW - Callose Synthase
KW - Expression
KW - Growth
KW - Regulation
KW - Salt
KW - qRT-PCR
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UR - https://www.mendeley.com/catalogue/23a86ced-e812-3648-8325-ddfdfecb739b/
U2 - 10.1016/j.jksus.2023.103049
DO - 10.1016/j.jksus.2023.103049
M3 - Article
AN - SCOPUS:85180788056
SN - 1018-3647
VL - 36
JO - Journal of King Saud University - Science
JF - Journal of King Saud University - Science
IS - 2
M1 - 103049
ER -