TY - JOUR
T1 - Full-length genome analysis of Čalovo strains of Batai orthobunyavirus (Bunyamwera serogroup)
T2 - IMPLICATIONS to taxonomy
AU - Dufkova, Lucie
AU - Pachler, Karin
AU - Kilian, Patrik
AU - Chrudimský, Tomáš
AU - Danielová, Vlasta
AU - Růžek, Daniel
AU - Nowotny, Norbert
N1 - Funding Information:
This study was partially funded by the EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext224 ( http://www.edenext.eu ). The contents of this publication are the sole responsibility of the authors and don’t necessarily reflect the views of the European Commission.
Funding Information:
We acknowledge the financial support by the Czech Science Foundation projects No. P502/11/2116 and No. 14-29256S, and the Admire Vet project No. CZ.1.05./2.1.00/01.006 (ED006/01/01).
PY - 2014/10
Y1 - 2014/10
N2 - Batai virus (BATV) is a poorly studied arthropod-borne virus belonging to the genus Orthobunyavirus (Bunyamwera serogroup) within the family Bunyaviridae. It has been associated with human influenza-like febrile illness in several Asian, African, and European countries. Čalovo virus (CVOV), isolated in 1960 in Slovakia, has been classified as BATV based on high antigenic similarity, and since then both CVOV and BATV were used as synonyms. In order to fully clarify the phylogenetic relationships between CVOV, BATV, and other members of the Bunyamwera serogroup, we performed whole genome sequencing of four CVOV strains isolated in Europe and phylogenetic analyses of all related viruses.The nucleocapsid protein, encoded by the S genomic segment, contains 233 amino acids, 60 of which, putatively critical for protein function, are conserved. Within the CVOV polyprotein encoded by the M genomic segment, putative cleavage sites, N-glycosylation sites, and seven transmembrane regions were identified. The RNA-dependent RNA polymerase, encoded by the L genome segment, exhibits conservation of the three regions known to be conserved among bunyavirus and arenavirus L proteins.Phylogenetic analyses of all three genomic segments of selected orthobunyaviruses clearly revealed that European and Asian/African strains of BATV are phylogenetically different and form two distinct lineages, indicating the existence of two different genotypes of BATV, tentatively named European genotype (with CVOV as a type strain) and Afro-Asian genotype (with BATV as a type strain) of BATV.
AB - Batai virus (BATV) is a poorly studied arthropod-borne virus belonging to the genus Orthobunyavirus (Bunyamwera serogroup) within the family Bunyaviridae. It has been associated with human influenza-like febrile illness in several Asian, African, and European countries. Čalovo virus (CVOV), isolated in 1960 in Slovakia, has been classified as BATV based on high antigenic similarity, and since then both CVOV and BATV were used as synonyms. In order to fully clarify the phylogenetic relationships between CVOV, BATV, and other members of the Bunyamwera serogroup, we performed whole genome sequencing of four CVOV strains isolated in Europe and phylogenetic analyses of all related viruses.The nucleocapsid protein, encoded by the S genomic segment, contains 233 amino acids, 60 of which, putatively critical for protein function, are conserved. Within the CVOV polyprotein encoded by the M genomic segment, putative cleavage sites, N-glycosylation sites, and seven transmembrane regions were identified. The RNA-dependent RNA polymerase, encoded by the L genome segment, exhibits conservation of the three regions known to be conserved among bunyavirus and arenavirus L proteins.Phylogenetic analyses of all three genomic segments of selected orthobunyaviruses clearly revealed that European and Asian/African strains of BATV are phylogenetically different and form two distinct lineages, indicating the existence of two different genotypes of BATV, tentatively named European genotype (with CVOV as a type strain) and Afro-Asian genotype (with BATV as a type strain) of BATV.
KW - Batai virus
KW - Orthobunyavirus
KW - Phylogenetic analysis
KW - Čalovo virus
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U2 - 10.1016/j.meegid.2014.07.005
DO - 10.1016/j.meegid.2014.07.005
M3 - Article
C2 - 25017655
AN - SCOPUS:84904914075
SN - 1567-1348
VL - 27
SP - 96
EP - 104
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -