TY - JOUR
T1 - Distribution of borna disease virus antigen and rna in tissues of naturally infected bicolored white-toothed shrews, crocidura leucodon, supporting their role as reservoir host species
AU - Puorger, M. E.
AU - Hilbe, M.
AU - Müller, J. P.
AU - Kolodziejek, J.
AU - Nowotny, N.
AU - Zlinszky, K.
AU - Ehrensperger, F.
N1 - Funding Information:
This investigation was financially supported by the Swiss Federal Veterinary Office, Bern, Switzerland.
PY - 2010
Y1 - 2010
N2 - Borna disease is a severe viral-induced disorder of the central nervous system of horses, sheep, and a few other animal species, occurring in certain areas of central Europe. Pathogenesis and epidemiology of natural Borna disease virus (BDV) infections are still not fully understood; several unique epidemiologic features, however, point toward the existence of BDV reservoir populations other than the final hosts. In this study, 69 mice and 12 shrews were trapped and examined. The virus distribution was investigated in detail in 2 BDV-positive bicolored white-toothed shrews, Crocidura leucodon, by immunohistochemistry and TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR). RT-PCR amplification products were sequenced, and the sequences were compared. These shrews had been collected in a BDV-endemic geographical region using live traps and did not show obvious clinical or pathological disease signs. BDV antigen and nucleic acid were identified in several organs, including the brain, mainly in nerve tissue and neurons, respectively, but also in parenchymal cells (eg, hepatocytes, Leydig cells) and epithelial cells, particularly of the respiratory and urogenital tract.
AB - Borna disease is a severe viral-induced disorder of the central nervous system of horses, sheep, and a few other animal species, occurring in certain areas of central Europe. Pathogenesis and epidemiology of natural Borna disease virus (BDV) infections are still not fully understood; several unique epidemiologic features, however, point toward the existence of BDV reservoir populations other than the final hosts. In this study, 69 mice and 12 shrews were trapped and examined. The virus distribution was investigated in detail in 2 BDV-positive bicolored white-toothed shrews, Crocidura leucodon, by immunohistochemistry and TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR). RT-PCR amplification products were sequenced, and the sequences were compared. These shrews had been collected in a BDV-endemic geographical region using live traps and did not show obvious clinical or pathological disease signs. BDV antigen and nucleic acid were identified in several organs, including the brain, mainly in nerve tissue and neurons, respectively, but also in parenchymal cells (eg, hepatocytes, Leydig cells) and epithelial cells, particularly of the respiratory and urogenital tract.
KW - Bicolored white-toothed shrew
KW - Borna disease virus
KW - Immunohistochemistry
KW - RT-PCR
KW - Reservoir species
KW - Reverse transcription polymerase chain re action
KW - Sequencing
KW - TaqMan real-time reverse transcription polymerase chain reaction
KW - Tissue distribution
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U2 - 10.1177/0300985809351849
DO - 10.1177/0300985809351849
M3 - Article
C2 - 20133953
AN - SCOPUS:77953995621
SN - 0300-9858
VL - 47
SP - 236
EP - 244
JO - Veterinary Pathology
JF - Veterinary Pathology
IS - 2
ER -