TY - JOUR
T1 - Detection of foot-and-mouth disease viruses from the A/AFRICA/G-I genotype in the Sultanate of Oman
AU - Al-Rawahi, Wafa Ahmed
AU - Elshafie, Elshafie Ibrahim
AU - Baqir, Senan
AU - Al-Ansari, Aliya
AU - Wadsworth, Jemma
AU - Hicks, Hayley M.
AU - Knowles, Nick J.
AU - Di Nardo, Antonello
AU - King, Donald P.
AU - Zientara, Stephan
AU - Al Salloom, Fajer
AU - Sangula, Abraham
AU - Bernelin-Cottet, Cindy
AU - Bakkali-Kassimi, Labib
AU - Al Riyami, Bahja
N1 - Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Rapid identification and characterization of circulating foot-and-mouth disease virus (FMDV) strains is crucial for effective disease control. In Oman, a few serological and molecular studies have been conducted to identify the strains of FMDV responsible for the outbreaks that have been occurring within the country. In this study, 13 oral epithelial tissue samples from cattle were collected from suspected cases of FMD in Ash Sharqiyah North, Al Batinah North, Dhofar and Ad Dhakhyilia governorates of Oman between 2018 and 2021. FMDV RNA was detected in all samples by real-time RT-PCR and viruses were isolated after one- or two-blind passages in the porcine Instituto Biologico-Rim Suino-2 cell line. Antigen capture ELISA characterized all isolates as serotype A and VP1 phylogenetic analysis placed all sequences within a single clade of the G-I genotype within the A/AFRICA topotype. These sequences shared the closest nucleotide identities to viruses circulating in Bahrain in 2021 (93.5% to 99.5%) and Kenya in 2017 (93.4% to 99.1%). To the best of our knowledge, this is the first time that A/AFRICA/G-I viruses have been detected in Oman. Together with the closely related viruses detected recently in Bahrain, these findings reinforce the importance of deploying effective quarantine control measures to minimize the risks of transboundary transmission of FMD associated with the importation of cattle from East Africa.
AB - Rapid identification and characterization of circulating foot-and-mouth disease virus (FMDV) strains is crucial for effective disease control. In Oman, a few serological and molecular studies have been conducted to identify the strains of FMDV responsible for the outbreaks that have been occurring within the country. In this study, 13 oral epithelial tissue samples from cattle were collected from suspected cases of FMD in Ash Sharqiyah North, Al Batinah North, Dhofar and Ad Dhakhyilia governorates of Oman between 2018 and 2021. FMDV RNA was detected in all samples by real-time RT-PCR and viruses were isolated after one- or two-blind passages in the porcine Instituto Biologico-Rim Suino-2 cell line. Antigen capture ELISA characterized all isolates as serotype A and VP1 phylogenetic analysis placed all sequences within a single clade of the G-I genotype within the A/AFRICA topotype. These sequences shared the closest nucleotide identities to viruses circulating in Bahrain in 2021 (93.5% to 99.5%) and Kenya in 2017 (93.4% to 99.1%). To the best of our knowledge, this is the first time that A/AFRICA/G-I viruses have been detected in Oman. Together with the closely related viruses detected recently in Bahrain, these findings reinforce the importance of deploying effective quarantine control measures to minimize the risks of transboundary transmission of FMD associated with the importation of cattle from East Africa.
KW - FMDV
KW - Genotype
KW - Oman
KW - Phylogeny
KW - Serotype A
KW - Transboundary
KW - Serogroup
KW - Cattle Diseases/epidemiology
KW - Foot-and-Mouth Disease/epidemiology
KW - Animals
KW - Oman/epidemiology
KW - Swine Diseases/epidemiology
KW - Cattle
KW - Swine
KW - Foot-and-Mouth Disease Virus
KW - Disease Outbreaks/veterinary
UR - http://www.scopus.com/inward/record.url?scp=85182174905&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85182174905&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/7ff29c59-022b-3484-87a6-3e541a6be502/
U2 - 10.1016/j.prevetmed.2023.106113
DO - 10.1016/j.prevetmed.2023.106113
M3 - Article
C2 - 38194859
AN - SCOPUS:85182174905
SN - 0167-5877
VL - 223
JO - Preventive Veterinary Medicine
JF - Preventive Veterinary Medicine
M1 - 106113
ER -