TY - JOUR
T1 - Chemical constituents and antioxidant activity of fresh leaves of Psidium guajava cultivated in Pakistan
AU - Begum, Sabira
AU - Ali, Syed Nawazish
AU - Tauseef, Saima
AU - Ali, Syed Tahir
AU - Hassan, Syed Imran
AU - Siddiqui, Bina Shaheen
AU - Ahmad, Aqeel
PY - 2014
Y1 - 2014
N2 - The in vitro antioxidant activities of the methanol extract of fresh leaves of Psidium guajava cultivated in Pakistan and its different fractions were evaluated using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging assay. The methanol extract, main ethyl acetate fraction and its polar sub fraction showed high free radical scavenging activity with EC50 11.72, 11.72 and 46.8 μg/mL respectively. The first two values are comparable with that of reference compound ascorbic acid (EC50 9.4 μg/mL). The known antioxidants gallic acid (1), methyl ferulate (2) and methyl p-E-coumarate (3) were isolated from the ethyl acetate insoluble fraction. Their structures were identified by mass, 1H- and 13C-NMR spectroscopy. Compounds 2 and 3 are reported for the first time from the genus Psidium.
AB - The in vitro antioxidant activities of the methanol extract of fresh leaves of Psidium guajava cultivated in Pakistan and its different fractions were evaluated using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging assay. The methanol extract, main ethyl acetate fraction and its polar sub fraction showed high free radical scavenging activity with EC50 11.72, 11.72 and 46.8 μg/mL respectively. The first two values are comparable with that of reference compound ascorbic acid (EC50 9.4 μg/mL). The known antioxidants gallic acid (1), methyl ferulate (2) and methyl p-E-coumarate (3) were isolated from the ethyl acetate insoluble fraction. Their structures were identified by mass, 1H- and 13C-NMR spectroscopy. Compounds 2 and 3 are reported for the first time from the genus Psidium.
KW - DPPH scavenging activity
KW - Gallic acid
KW - Methyl ferulate
KW - Methyl p-E-coumarate
KW - Psidium guajava
KW - Reactive oxygen species
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M3 - Article
AN - SCOPUS:84899107335
SN - 0253-5106
VL - 36
SP - 119
EP - 122
JO - Journal of the Chemical Society of Pakistan
JF - Journal of the Chemical Society of Pakistan
IS - 1
ER -