TY - JOUR
T1 - Are tubes containing sodium fluoride still needed for the measurement of blood glucose in hospital laboratory practice?
AU - Al-Kharusi, Amira
AU - Al-Lawati, Nuha
AU - Al-Kindi, Manal
AU - Mula-Abed, Waad Allah
N1 - Publisher Copyright:
© 2015, Oman Medical Specialty Board All rights reserved.
PY - 2014
Y1 - 2014
N2 - Objectives: To compare glucose values obtained using sodium flouride (NaF) tubes and serum separation tubes (SST) in a tertiary care hospital laboratory setting.Methods: This study was conducted at the Clinical Biochemistry Laboratory, Royal Hospital, Oman. During the study period (1 September–30 November 2013), 50 pairs (one NaF tube and one SST) of patient’s blood specimens were randomly collected. Following separation of plasma (NaF tubes) and serum (SST), glucose concentrations were measured by hexokinase assay using the Architect c8000. Fifteen pairs of these tubes were kept in the refrigerator at 4°C and plasma/serum glucose concentrations were measured daily up to seven days after collection.Results: Comparing plasma (NaF) and serum (SST) results of glucose values (n=50) showed an average difference of 0.00mmol/L (range -0.60 to +0.60mmol/L). Bland Altman analysis gave a non-significant constant bias of 0.10 ±0.195mmol/L (bias ±SD). Pearson correlation between plasma (NaF) and serum (SST) glucose concentrations revealed a significant correlation approaching unity with r2= 0.9991. No significant differences in glucose values were noted for both plasma and serum in 15 pairs of NaF and SST tubes when analyzed seven days following refrigeration. Hemolysis was observed in five (10%) NaF tubes compared with two (4%) SST.Conclusion: There is no difference in glucose values collected from plasma NaF tubes or serum SST, and so SST can be used in hospital laboratory settings as there are practical advantages, including cost-effectiveness and reduction in blood volume drawn when utilizing these tubes for glucose and other tests from a single blood collection tube.
AB - Objectives: To compare glucose values obtained using sodium flouride (NaF) tubes and serum separation tubes (SST) in a tertiary care hospital laboratory setting.Methods: This study was conducted at the Clinical Biochemistry Laboratory, Royal Hospital, Oman. During the study period (1 September–30 November 2013), 50 pairs (one NaF tube and one SST) of patient’s blood specimens were randomly collected. Following separation of plasma (NaF tubes) and serum (SST), glucose concentrations were measured by hexokinase assay using the Architect c8000. Fifteen pairs of these tubes were kept in the refrigerator at 4°C and plasma/serum glucose concentrations were measured daily up to seven days after collection.Results: Comparing plasma (NaF) and serum (SST) results of glucose values (n=50) showed an average difference of 0.00mmol/L (range -0.60 to +0.60mmol/L). Bland Altman analysis gave a non-significant constant bias of 0.10 ±0.195mmol/L (bias ±SD). Pearson correlation between plasma (NaF) and serum (SST) glucose concentrations revealed a significant correlation approaching unity with r2= 0.9991. No significant differences in glucose values were noted for both plasma and serum in 15 pairs of NaF and SST tubes when analyzed seven days following refrigeration. Hemolysis was observed in five (10%) NaF tubes compared with two (4%) SST.Conclusion: There is no difference in glucose values collected from plasma NaF tubes or serum SST, and so SST can be used in hospital laboratory settings as there are practical advantages, including cost-effectiveness and reduction in blood volume drawn when utilizing these tubes for glucose and other tests from a single blood collection tube.
KW - Blood glucose
KW - Diabetes mellitus
KW - Serum separator tubes (SST)
KW - Sodium fluoride
UR - http://www.scopus.com/inward/record.url?scp=84922220392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922220392&partnerID=8YFLogxK
U2 - 10.5001/omj.2014.109
DO - 10.5001/omj.2014.109
M3 - Article
C2 - 25584156
AN - SCOPUS:84922220392
SN - 1999-768X
VL - 29
SP - 404
EP - 407
JO - Oman Medical Journal
JF - Oman Medical Journal
IS - 6
ER -