TY - JOUR
T1 - Antitumor and antioxidant activities of polysaccharides from the seaweed Durvillaea antarctica
AU - Roberto T., Abdala Díaz
AU - Virginia, Casas Arrojo
AU - Ángeles, Arrojo Agudo María
AU - Casimiro, Cárdenas García
AU - Claudia, Pérez Manríquez
AU - Eduardo, Uribe
AU - Félix, Álvarez Gómez
AU - Nathalie, Korbee
AU - Félix L., Figueroa
AU - Sergey, Dobretsov
N1 - Publisher Copyright:
© 2023 John Wiley & Sons A/S.
PY - 2024/1
Y1 - 2024/1
N2 - The present study was carried out to determine the antitumor and antioxidant activities of the seaweed Durvillaea antarctica. Extraction and purification of polysaccharides from D. antarctica were performed. They were characterized by FT-IR and GC–MS, identifying isomers of arabinose, fucose, mannose, and galactose. The antioxidant capacity of polysaccharides was analyzed using the ABTS method (14.3 ± 0.5 μmol TE g−1 PS) and the DPPH method (21.82 ± 0.32 μmol TE g−1 PS). The antitumor capacity of polysaccharides was studied by MTT colorimetric assays in human leukemia, colon, breast, and lung cancer cell lines, obtaining the lowest IC50 in colon cancer (19.99 μg mL−1). In the line of healthy human gingival fibroblasts (HGF-1), an IC50 of 444.39 μg mL−1 was obtained. Flow cytometry in the HL60 cell line showed that polysaccharides at concentrations higher than IC50 inhibited cell proliferation, demonstrating a possible antitumor capacity in vitro. In the proteomic analysis with HGF-1, nine proteins involved in different biological processes were identified. In conclusion, polysaccharides from D. antarctica could be considered powerful nutraceuticals, mainly against colon cancer.
AB - The present study was carried out to determine the antitumor and antioxidant activities of the seaweed Durvillaea antarctica. Extraction and purification of polysaccharides from D. antarctica were performed. They were characterized by FT-IR and GC–MS, identifying isomers of arabinose, fucose, mannose, and galactose. The antioxidant capacity of polysaccharides was analyzed using the ABTS method (14.3 ± 0.5 μmol TE g−1 PS) and the DPPH method (21.82 ± 0.32 μmol TE g−1 PS). The antitumor capacity of polysaccharides was studied by MTT colorimetric assays in human leukemia, colon, breast, and lung cancer cell lines, obtaining the lowest IC50 in colon cancer (19.99 μg mL−1). In the line of healthy human gingival fibroblasts (HGF-1), an IC50 of 444.39 μg mL−1 was obtained. Flow cytometry in the HL60 cell line showed that polysaccharides at concentrations higher than IC50 inhibited cell proliferation, demonstrating a possible antitumor capacity in vitro. In the proteomic analysis with HGF-1, nine proteins involved in different biological processes were identified. In conclusion, polysaccharides from D. antarctica could be considered powerful nutraceuticals, mainly against colon cancer.
KW - anticancer activity
KW - antioxidant activity
KW - Durvillaea antarctica
KW - FT-IR spectroscopy
KW - polysaccharides
KW - seaweed
KW - Antioxidants/pharmacology
KW - Humans
KW - Polysaccharides/pharmacology
KW - Spectroscopy, Fourier Transform Infrared
KW - Seaweed
KW - Proteomics
KW - Colonic Neoplasms/drug therapy
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U2 - 10.1111/cbdd.14392
DO - 10.1111/cbdd.14392
M3 - Article
C2 - 37945521
AN - SCOPUS:85176229296
SN - 1747-0277
VL - 103
JO - Chemical Biology and Drug Design
JF - Chemical Biology and Drug Design
IS - 1
M1 - e14392
ER -