TY - JOUR
T1 - Anti-pathogenic, anti-diabetic, anti-inflammatory, antioxidant, and wound healing efficacy of Datura metel L. leaves
AU - Prasathkumar, Murugan
AU - Anisha, Salim
AU - Khusro, Ameer
AU - Mohamed Essa, Musthafa
AU - Babu Chidambaram, Saravana
AU - Walid Qoronfleh, M.
AU - Sadhasivam, Subramaniam
AU - Umar Khayam Sahibzada, Muhammad
AU - Alghamdi, Saad
AU - Almehmadi, Mazen
AU - Abdulaziz, Osama
AU - Khandaker, Mayeen Uddin
AU - Faruque, Mohammad Rashed Iqbal
AU - Emran, Talha Bin
N1 - Funding Information:
The author, Subramaniam Sadhasivam acknowledges DBT, India for the financial support provided by the Ramalingaswami Re-entry fellowship (Order No.BT/RLF/Reentry/55/2013). The authors thank the DST FIST (SR/FST/LS-I/2017/10) supported Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India for providing the common instrumentation facilities to carry out this work. This work was supported by the Research Universiti Grant, Geran Universiti Penyelidikan (GUP), code: 2021-074. The authors like to thank Taif University, Taif, Saudi Arabia for their support (Taif University Researcher Supporting Project Number: TURSP-2020/80).
Funding Information:
The author, Subramaniam Sadhasivam acknowledges DBT, India for the financial support provided by the Ramalingaswami Re-entry fellowship (Order No. BT/RLF/Reentry/55/2013 ). The authors thank the DST FIST ( SR/FST/LS-I/2017/10 ) supported Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India for providing the common instrumentation facilities to carry out this work. This work was supported by the Research Universiti Grant, Geran Universiti Penyelidikan (GUP) , code: 2021-074 . The authors like to thank Taif University, Taif, Saudi Arabia for their support (Taif University Researcher Supporting Project Number: TURSP-2020/80 ).
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/9
Y1 - 2022/9
N2 - Datura metel L. is an important medicinal plant of Solanaceae family which has extensive pharmacological properties. The present investigation was aimed to identify the presence of phytoconstituents and assess in vitro antibacterial, anti-biofilm, anti-diabetic, anti-inflammatory, antioxidant, cytotoxicity, and wound healing efficacy of D. metel leaves extract. Among different solvent extracts, methanolic extract showed higher amount of phenolic (124.61 ± 0.68 mg GAE/g), alkaloid (88.77 ± 1.01 mg AE/g), flavonoids (42.24 ± 0.18 mg QE/g), and tannins contents (38.72 ± 0.51 mg GAE/g). The extract exhibited not only significantly (P < 0.05) different antibacterial activities against pathogens tested but also showed maximum biofilm inhibition of 94, 88, and 92% against B. subtilis, MRSA, and E. coli, respectively. Anti-diabetic assay depicted 22.55 ± 0.62–79.41 ± 1.13% and 24.31 ± 1.47–72.59 ± 0.22% of α-amylase and α-glucosidase inhibition abilities of methanolic extract, respectively at varied concentrations. The methanolic extract showed potential anti-inflammatory effect (P < 0.05) by showing 28.11 ± 0.13, 34.94 ± 1.11, 55.73 ± 0.42, 73.28 ± 0.72, and 92.62 ± 1.33% of inhibition of protein denaturation at different concentrations with an IC50 value of 52.45 µg/mL. The extract revealed significant (P < 0.05) rate of ABTS scavenging, DPPH degradation, and reducing power assay in a concentration dependent manner. The cytotoxicity assay was demonstrated on L929 mouse fibroblast cell line and found > 90% of cell viability in the presence of methanolic extract, thereby indicating its non-toxicity effect. Wound healing assay indicated that methanolic extract at 50 µg/mL closed 100% of wound gap after 24 h with high rate of migration and proliferation. Furthermore, GC–MS chromatogram revealed the presence of several components in methanolic extract, including neophytadiene, hexadecanoic acid, and hentriacontane as principal phytoconstituents. In conclusion, methanolic extract of D. metel leaves could be used as potent therapeutic agent not only for treating metabolic diseases but also superficial chronic diabetic wounds.
AB - Datura metel L. is an important medicinal plant of Solanaceae family which has extensive pharmacological properties. The present investigation was aimed to identify the presence of phytoconstituents and assess in vitro antibacterial, anti-biofilm, anti-diabetic, anti-inflammatory, antioxidant, cytotoxicity, and wound healing efficacy of D. metel leaves extract. Among different solvent extracts, methanolic extract showed higher amount of phenolic (124.61 ± 0.68 mg GAE/g), alkaloid (88.77 ± 1.01 mg AE/g), flavonoids (42.24 ± 0.18 mg QE/g), and tannins contents (38.72 ± 0.51 mg GAE/g). The extract exhibited not only significantly (P < 0.05) different antibacterial activities against pathogens tested but also showed maximum biofilm inhibition of 94, 88, and 92% against B. subtilis, MRSA, and E. coli, respectively. Anti-diabetic assay depicted 22.55 ± 0.62–79.41 ± 1.13% and 24.31 ± 1.47–72.59 ± 0.22% of α-amylase and α-glucosidase inhibition abilities of methanolic extract, respectively at varied concentrations. The methanolic extract showed potential anti-inflammatory effect (P < 0.05) by showing 28.11 ± 0.13, 34.94 ± 1.11, 55.73 ± 0.42, 73.28 ± 0.72, and 92.62 ± 1.33% of inhibition of protein denaturation at different concentrations with an IC50 value of 52.45 µg/mL. The extract revealed significant (P < 0.05) rate of ABTS scavenging, DPPH degradation, and reducing power assay in a concentration dependent manner. The cytotoxicity assay was demonstrated on L929 mouse fibroblast cell line and found > 90% of cell viability in the presence of methanolic extract, thereby indicating its non-toxicity effect. Wound healing assay indicated that methanolic extract at 50 µg/mL closed 100% of wound gap after 24 h with high rate of migration and proliferation. Furthermore, GC–MS chromatogram revealed the presence of several components in methanolic extract, including neophytadiene, hexadecanoic acid, and hentriacontane as principal phytoconstituents. In conclusion, methanolic extract of D. metel leaves could be used as potent therapeutic agent not only for treating metabolic diseases but also superficial chronic diabetic wounds.
KW - Bioactive properties
KW - Datura metel L.
KW - Methanolic extract
KW - Therapeutics
KW - Wound healing
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U2 - 10.1016/j.arabjc.2022.104112
DO - 10.1016/j.arabjc.2022.104112
M3 - Article
AN - SCOPUS:85134595799
SN - 1878-5352
VL - 15
JO - Arabian Journal of Chemistry
JF - Arabian Journal of Chemistry
IS - 9
M1 - 104112
ER -
