TY - JOUR
T1 - A molecular epidemiological investigation of contagious caprine pleuropneumonia in goats and captive Arabian sand gazelle (Gazella marica) in Oman
AU - Ali, Haytham
AU - El-Neweshy, Mahmoud
AU - Al Mawly, Julanda
AU - Heller, Martin
AU - Weber, Michael
AU - Schnee, Christiane
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/4/25
Y1 - 2024/4/25
N2 - Background: Contagious caprine pleuropneumonia (CCPP) is a fatal WOAH-listed, respiratory disease in small ruminants with goats as primary hosts that is caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). Twelve CCPP outbreaks were investigated in 11 goat herds and a herd of captive Arabian sand gazelle (Gazella marica) in four Omani governorates by clinical pathological and molecular analysis to compare disease manifestation and Mccp genetic profiles in goats and wild ungulates. Results: The CCPP forms in diseased and necropsied goats varied from peracute (5.8%), acute (79.2%) and chronic (4.5%) while all of the five necropsied gazelles showed the acute form based on the clinical picture, gross and histopathological evaluation. Colonies of Mccp were recovered from cultured pleural fluid, but not from lung tissue samples of one gazelle and nine goats and all the isolates were confirmed by Mccp-specific real time PCR. Whole genome-single nucleotide polymorphism (SNP) analysis was performed on the ten isolates sequenced in this study and twenty sequences retrieved from the Genbank database. The Mccp strains from Oman clustered all in phylogroup A together with strains from East Africa and one strain from Qatar. A low variability of around 125 SNPs was seen in the investigated Omani isolates from both goats and gazelles indicating mutual transmission of the pathogen between wildlife and goats. Conclusion: Recent outbreaks of CCPP in Northern Oman are caused by Mccp strains of the East African Phylogroup A which can infect goats and captive gazelles likewise. Therefore, wild and captive ungulates should be considered as reservoirs and included in CCPP surveillance measures.
AB - Background: Contagious caprine pleuropneumonia (CCPP) is a fatal WOAH-listed, respiratory disease in small ruminants with goats as primary hosts that is caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). Twelve CCPP outbreaks were investigated in 11 goat herds and a herd of captive Arabian sand gazelle (Gazella marica) in four Omani governorates by clinical pathological and molecular analysis to compare disease manifestation and Mccp genetic profiles in goats and wild ungulates. Results: The CCPP forms in diseased and necropsied goats varied from peracute (5.8%), acute (79.2%) and chronic (4.5%) while all of the five necropsied gazelles showed the acute form based on the clinical picture, gross and histopathological evaluation. Colonies of Mccp were recovered from cultured pleural fluid, but not from lung tissue samples of one gazelle and nine goats and all the isolates were confirmed by Mccp-specific real time PCR. Whole genome-single nucleotide polymorphism (SNP) analysis was performed on the ten isolates sequenced in this study and twenty sequences retrieved from the Genbank database. The Mccp strains from Oman clustered all in phylogroup A together with strains from East Africa and one strain from Qatar. A low variability of around 125 SNPs was seen in the investigated Omani isolates from both goats and gazelles indicating mutual transmission of the pathogen between wildlife and goats. Conclusion: Recent outbreaks of CCPP in Northern Oman are caused by Mccp strains of the East African Phylogroup A which can infect goats and captive gazelles likewise. Therefore, wild and captive ungulates should be considered as reservoirs and included in CCPP surveillance measures.
KW - Contagious caprine pleuropneumonia
KW - Mycoplasma capricolum ssp. capripneumoniae
KW - Oman
KW - Whole genome SNP analysis
KW - Goats
KW - Mycoplasma capricolum/genetics
KW - Goat Diseases/epidemiology
KW - Phylogeny
KW - Antelopes
KW - Pleuropneumonia, Contagious/epidemiology
KW - Animals
KW - Oman/epidemiology
KW - Molecular Epidemiology
KW - Polymorphism, Single Nucleotide
KW - Disease Outbreaks/veterinary
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UR - https://www.mendeley.com/catalogue/db5ee8d8-d822-347d-b491-6ef293d38dbc/
U2 - 10.1186/s12917-024-03969-1
DO - 10.1186/s12917-024-03969-1
M3 - Article
C2 - 38664764
AN - SCOPUS:85191341233
SN - 1746-6148
VL - 20
SP - 155
JO - BMC Veterinary Research
JF - BMC Veterinary Research
IS - 1
M1 - 155
ER -