Use of germ-free animal models in microbiota-related research

Maha Al-Asmakh*, Fahad Zadjali

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

160 Citations (Scopus)


The large intestine is a home for trillions of microbiota, which confer many benefits on the host, including production of vitamins, absorption of nutrients, pathogen displacement, and development of the immune system. For several decades, germ-free animals have been used to study the interaction between the host and its microbiota. This minireview describes the technical aspects for establishing and maintaining germ-free animals and highlights the advantages and disadvantages for germ-free animals as experimental models.

Original languageEnglish
Article numberA001
Pages (from-to)1583-1588
Number of pages6
JournalJournal of Microbiology and Biotechnology
Issue number10
Publication statusPublished - 2015


  • Germ-free animals
  • Germ-free mice
  • Gnotobiotic
  • Isolator technology
  • Microbiota

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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