Potential application of crispr/cas9 system to engineer abiotic stress tolerance in plants

Temoor Ahmed, Muhammad Noman, Muhammad Shahid*, Sher Muhammad, Muhammad Tahir Ul Qamar, Md Arshad Ali, Awais Maqsood, Rahila Hafeez, Solabomi Olaitan Ogunyemi, Bin Li*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

6 Citations (Scopus)


Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly inters-paced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review high-lighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.

Original languageEnglish
Pages (from-to)861-877
Number of pages17
JournalProtein and Peptide Letters
Issue number8
Publication statusPublished - Aug 2021


  • Abiotic stresses
  • CRISPR/Cas9
  • Crop improvement
  • Genome editing
  • SgRNA
  • Transcription

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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