International review of blood donation nucleic acid amplification testing

Helen M. Faddy*, Carla Osiowy, Brian Custer, Michael Busch, Susan L. Stramer, Opeyemi Adesina, Thijs van de Laar, Wai Chiu Tsoi, Claire Styles, Phil Kiely, Angelo Margaritis, So Yong Kwon, Yan Qiu, Xuelian Deng, Antoine Lewin, Signe Winther Jørgensen, Christian Erikstrup, David Juhl, Silvia Sauleda, Bernardo Armando Camacho RodriguezLisbeth Jennifer Catherine Soto Coral, Paula Andrea Gaviria García, Sineenart Oota, Sheila F. O'Brien, Silvano Wendel, Emma Castro, Laura Navarro Pérez, Heli Harvala, Katy Davison, Claire Reynolds, Lisa Jarvis, Piotr Grabarczyk, Aneta Kopacz, Magdalena Łętowska, Niamh O'Flaherty, Fiona Young, Padraig Williams, Lisa Burke, Sze Sze Chua, An Muylaert, Isabel Page, Ann Jones, Christoph Niederhauser, Marion Vermeulen, Syria Laperche, Pierre Gallian, Salam Sawadogo, Masahiro Satake, Ahmad Gharehbaghian, Marcelo Addas-Carvalho, Sebastián Blanco, Sandra V. Gallego, Axel Seltsam, Marijke Weber-Schehl, Arwa Z. Al-Riyami, Khuloud Al Maamari, Fatma Ba Alawi, Hem Chandra Pandey, Dora Mbanya, Rochele Azevedo França, Richard Charlewood

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


Background and Objectives: Nucleic acid amplification testing (NAT), in blood services context, is used for the detection of viral and parasite nucleic acids to reduce transfusion-transmitted infections. This project reviewed NAT for screening blood donations globally. Materials and Methods: A survey on NAT usage, developed by the International Society of Blood Transfusion Working Party on Transfusion-transmitted Infectious Diseases (ISBT WP-TTID), was distributed through ISBT WP-TTID members. Data were analysed using descriptive statistics. Results: Forty-three responses were received from 32 countries. Increased adoption of blood donation viral screening by NAT was observed over the past decade. NAT-positive donations were detected for all viruses tested in 2019 (proportion of donations positive by NAT were 0.0099% for human immunodeficiency virus [HIV], 0.0063% for hepatitis C virus [HCV], 0.0247% for hepatitis B virus [HBV], 0.0323% for hepatitis E virus [HEV], 0.0014% for West Nile virus [WNV] and 0.00005% for Zika virus [ZIKV]). Globally, over 3100 NAT-positive donations were identified as NAT yield or solely by NAT in 2019 and over 22,000 since the introduction of NAT, with HBV accounting for over half. NAT-positivity rate was higher in first-time donors for all viruses tested except WNV. During 2019, a small number of participants performed NAT for parasites (Trypanosoma cruzi, Babesia spp., Plasmodium spp.). Conclusion: This survey captures current use of blood donation NAT globally. There has been increased NAT usage over the last decade. It is clear that NAT contributes to improving blood transfusion safety globally; however, there is a need to overcome economic barriers for regions/countries not performing NAT.

Original languageEnglish
Pages (from-to)315-325
Number of pages11
JournalVox Sanguinis
Issue number4
Publication statusPublished - Feb 23 2024


  • blood
  • NAT
  • safety
  • transfusion
  • TTI
  • virus
  • Hepatitis B/diagnosis
  • Humans
  • Zika Virus
  • Zika Virus Infection
  • Nucleic Acid Amplification Techniques
  • Nucleic Acids
  • Transfusion Reaction
  • Blood Donation
  • Blood Donors
  • Hepatitis B virus/genetics

ASJC Scopus subject areas

  • Hematology

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