Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal

M. S. Shahid; B. J. Pudashini; G. B. Khatri-Chhetri; Keiko T. Natsuaki; Rob W. Briddon

doi:10.1007/s13313-019-00630-6

Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal

M. S. Shahid^*, B. J. Pudashini, G. B. Khatri-Chhetri, Keiko T. Natsuaki, Rob W. Briddon

^*Corresponding author for this work

Crop Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Lima bean (Phaseolus lunatus) plants exhibiting yellow mosaic and stunted growth symptoms, typical of begomovirus infection, were collected in Rampur city Nepal. Symptomatic plants were shown to be infected by a monopartite begomovirus in association with a betasatellite. The two begomovirus isolates associated with the disease showed 98.7% nucleotide sequence identity to previously characterized isolates of the recently identified begomovirus species Pea leaf distortion virus (PLDV) from Nepal. The associated betasatellite isolates showed highest nucleotide sequence identity at 99% to Ludwigia leaf distortion betasatellite (LuLDB) from Nepal. This is the first identification of PLDV and LuLDB infecting the grain legume Phaseolus lunatus.

Original language	English
Pages (from-to)	309-312
Number of pages	4
Journal	Australasian Plant Pathology
Volume	48
Issue number	3
DOIs	https://doi.org/10.1007/s13313-019-00630-6
Publication status	Published - May 1 2019

Keywords

Begomovirus
Betasatellite
Geminivirus
Phaseolus lunatus

ASJC Scopus subject areas

Plant Science

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10.1007/s13313-019-00630-6

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@article{1bccfa3cf60f4cca872582a085060d21,

title = "Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal",

abstract = "Lima bean (Phaseolus lunatus) plants exhibiting yellow mosaic and stunted growth symptoms, typical of begomovirus infection, were collected in Rampur city Nepal. Symptomatic plants were shown to be infected by a monopartite begomovirus in association with a betasatellite. The two begomovirus isolates associated with the disease showed 98.7% nucleotide sequence identity to previously characterized isolates of the recently identified begomovirus species Pea leaf distortion virus (PLDV) from Nepal. The associated betasatellite isolates showed highest nucleotide sequence identity at 99% to Ludwigia leaf distortion betasatellite (LuLDB) from Nepal. This is the first identification of PLDV and LuLDB infecting the grain legume Phaseolus lunatus.",

keywords = "Begomovirus, Betasatellite, Geminivirus, Phaseolus lunatus",

author = "Shahid, {M. S.} and Pudashini, {B. J.} and Khatri-Chhetri, {G. B.} and Natsuaki, {Keiko T.} and Briddon, {Rob W.}",

note = "Funding Information: Acknowledgements RWB was supported by the Higher Education Commission, Govt. of Pakistan, under the BForeign Faculty Hiring Program^.",

year = "2019",

month = may,

day = "1",

doi = "10.1007/s13313-019-00630-6",

language = "English",

volume = "48",

pages = "309--312",

journal = "Australasian Plant Pathology",

issn = "0815-3191",

publisher = "Springer Netherlands",

number = "3",

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TY - JOUR

T1 - Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal

AU - Shahid, M. S.

AU - Pudashini, B. J.

AU - Khatri-Chhetri, G. B.

AU - Natsuaki, Keiko T.

AU - Briddon, Rob W.

N1 - Funding Information: Acknowledgements RWB was supported by the Higher Education Commission, Govt. of Pakistan, under the BForeign Faculty Hiring Program^.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Lima bean (Phaseolus lunatus) plants exhibiting yellow mosaic and stunted growth symptoms, typical of begomovirus infection, were collected in Rampur city Nepal. Symptomatic plants were shown to be infected by a monopartite begomovirus in association with a betasatellite. The two begomovirus isolates associated with the disease showed 98.7% nucleotide sequence identity to previously characterized isolates of the recently identified begomovirus species Pea leaf distortion virus (PLDV) from Nepal. The associated betasatellite isolates showed highest nucleotide sequence identity at 99% to Ludwigia leaf distortion betasatellite (LuLDB) from Nepal. This is the first identification of PLDV and LuLDB infecting the grain legume Phaseolus lunatus.

AB - Lima bean (Phaseolus lunatus) plants exhibiting yellow mosaic and stunted growth symptoms, typical of begomovirus infection, were collected in Rampur city Nepal. Symptomatic plants were shown to be infected by a monopartite begomovirus in association with a betasatellite. The two begomovirus isolates associated with the disease showed 98.7% nucleotide sequence identity to previously characterized isolates of the recently identified begomovirus species Pea leaf distortion virus (PLDV) from Nepal. The associated betasatellite isolates showed highest nucleotide sequence identity at 99% to Ludwigia leaf distortion betasatellite (LuLDB) from Nepal. This is the first identification of PLDV and LuLDB infecting the grain legume Phaseolus lunatus.

KW - Begomovirus

KW - Betasatellite

KW - Geminivirus

KW - Phaseolus lunatus

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U2 - 10.1007/s13313-019-00630-6

DO - 10.1007/s13313-019-00630-6

M3 - Article

AN - SCOPUS:85064956679

SN - 0815-3191

VL - 48

SP - 309

EP - 312

JO - Australasian Plant Pathology

JF - Australasian Plant Pathology

IS - 3

ER -

Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal

Abstract

Keywords

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