Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Summar A. Naqvi; M. M. Khan; M. Shahid; M. J. Jaskani; Iqrar A. Khan; Mohammad Zuber; Khalid Mahmood Zia

doi:10.1016/j.carbpol.2010.08.031

Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Summar A. Naqvi, M. M. Khan, M. Shahid, M. J. Jaskani, Iqrar A. Khan, Mohammad Zuber, Khalid Mahmood Zia

Crop Sciences

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40 Citations (SciVal)

Abstract

Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04; maltose 3.23-4.31; glucosamine 0.017-0.289; d-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg^-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.31-2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g^-1 energy. The comparative characterization of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

Original language	English
Pages (from-to)	623-628
Number of pages	6
Journal	Carbohydrate Polymers
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/j.carbpol.2010.08.031
Publication status	Published - Jan 10 2011

Keywords

Citrus
Mucilage
Polysaccharides
Rootstocks
Seed

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2010.08.031

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@article{d338730976fe4ee8b79ecbaffdda6e7b,

title = "Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks",

abstract = "Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04; maltose 3.23-4.31; glucosamine 0.017-0.289; d-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.31-2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterization of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.",

keywords = "Citrus, Mucilage, Polysaccharides, Rootstocks, Seed",

author = "Naqvi, {Summar A.} and Khan, {M. M.} and M. Shahid and Jaskani, {M. J.} and Khan, {Iqrar A.} and Mohammad Zuber and Zia, {Khalid Mahmood}",

note = "Funding Information: We acknowledge the Ministry of Science and Technology, Government of Pakistan, for their support to carry out this research work under the project entitled “Establishment of a Modern Citrus Nursery of Certified Plants through Sanitation Techniques”.",

year = "2011",

month = jan,

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doi = "10.1016/j.carbpol.2010.08.031",

language = "English",

volume = "83",

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journal = "Carbohydrate Polymers",

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TY - JOUR

T1 - Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

AU - Naqvi, Summar A.

AU - Khan, M. M.

AU - Shahid, M.

AU - Jaskani, M. J.

AU - Khan, Iqrar A.

AU - Zuber, Mohammad

AU - Zia, Khalid Mahmood

N1 - Funding Information: We acknowledge the Ministry of Science and Technology, Government of Pakistan, for their support to carry out this research work under the project entitled “Establishment of a Modern Citrus Nursery of Certified Plants through Sanitation Techniques”.

PY - 2011/1/10

Y1 - 2011/1/10

N2 - Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04; maltose 3.23-4.31; glucosamine 0.017-0.289; d-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.31-2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterization of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

AB - Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04; maltose 3.23-4.31; glucosamine 0.017-0.289; d-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.31-2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterization of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

KW - Citrus

KW - Mucilage

KW - Polysaccharides

KW - Rootstocks

KW - Seed

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DO - 10.1016/j.carbpol.2010.08.031

M3 - Article

AN - SCOPUS:78349304038

SN - 0144-8617

VL - 83

SP - 623

EP - 628

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 2

ER -

Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Abstract

Keywords

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