Addition of Gallic Acid Overcomes Resistance to Cisplatin in Ovarian Cancer Cell Lines

Najwa Al Balushi, Syed Imran Hassan*, Nada Abdullah, Buthaina Al Dhahli, Shadia Al Bahlani, Ikhlas Ahmed, Benjamin K. Tsang, Sergey Dobretsov, Yahya Tamimi, Ikram A. Burney

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: Ovarian cancer is one of the leading causes of cancer-related mortality in women, and is often associated with drug resistance. Therefore, finding effective drugs, including naturally derived compounds, is urgently needed. Herein, we aimed to test the anti-cancer potential of gallic acid monohydrate (GA) and its congeners on cisplatin-sensitive (A2780S), and resistant (A2780CP) ovarian cancer and normal ovarian (HOSE6-3) cell lines. Methods: Cytotoxicity was assessed by AlamarBlue and CCK08 assays by exposing cells to different concentrations of cisplatin (0-21μg/mL), GA and its congeners (0-100μg/mL), and a combination of GA and cisplatin. Apoptosis was estimated by Hoechst stain and monitoring the relative RNA expression of the apoptotic effector caspase-3 using qRT-PCR. Results: GA decreased cell viability in a concentration-dependent manner in all cell lines, with an IC50 of 19.39μg/mL (A2780S), 35.59 μg/ mL (A2780CP), and 49.32μg/mL (HOSE6-3). GA displayed higher cytotoxicity than its congeners. An apoptotic rate estimation of approximately 20% and 30% was obtained in A2780S and A2780CP. While the cytotoxicity observed with cisplatin and GA was comparable, combining the two enhanced the cytotoxicity significantly, especially in the A2780CP cell line (p<0.05). Conclusion: These data suggest that GA may help overcome the resistance. Hence, the cytotoxic effects of GA, especially on chemo-resistant ovarian cancer cells merit further investigation.

Original languageEnglish
Pages (from-to)2661-2669
Number of pages9
JournalAsian Pacific Journal of Cancer Prevention
Issue number8
Publication statusPublished - 2022


  • Chemo
  • Cisplatin
  • Cytotoxicity
  • Gallic acid
  • Ovarian cancer
  • Resistance

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Oncology
  • Cancer Research
  • Epidemiology

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