Reliability of carbapenem inactivation method (CIM) and modified carbapenem inactivation method (mCIM) for detection of OXA-48-like and NDM-1

Meher Rizvi*, Hiba Sami, Mohammad Azam, Dhoha Ben Khalid, Zaaima AL Jabri, Fatima Khan, Asfia Sultan, Anuradha Singh, Nusrat Perween, Munawer AL Quraini, Zakariya AL Muharrmi, Syed G. Rizvi

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

2 الاقتباسات (SciVal)


Purpose: Carbapenem inactivation method (CIM) and modified carbapenem inactivation method (mCIM) were recently developed for rapid detection of carbapenemase producing Gram negative bacilli (CP-GNB). In this study we compared the ability of modified Hodge test (MHT), CIM and mCIM to identify CP-GNB in Oman and India. Methods: Fifty fully characterized and genotyped CP-GNB (26 OXA-48-like, 2 NDM-1 from Oman and 22 NDM-1 from India) and 8 AmpC as controls in India were subjected to MHT, CIM, mCIM and mCIM with in-house modifications. Wilcoxon paired test and receiver operating characteristics (ROC) were utilised for statistical analysis. Results: Isolates were predominantly OXA-48-like genes producing Klebsiella pneumoniae from Oman and NDM-1 producing Escherichia coli from India. MHT was positive in all except one OXA-48-like producers and in 70.8 ​% of the NDM-1 isolates. The sensitivity of CIM in detecting 0XA-48 like and NDM-1 carbapenemases were 39.2% and 87.5% respectively. mCIM at 4 ​h detected 92.3 ​% and 79.1% of 0XA-48 and NDM-1 respectively. Using receiver operative characteristics (ROC), highest sensitivity and specificity for detection of OXA-48-like was obtained by mCIM at 4 ​h at cut off 17 ​mm while for NDM-1 CIM was the test of choice at 16 ​mm. Conclusion: CIM and mCIM are simple, cheap and easy tests to perform. CIM gave excellent results with NDM1 strains while it was quite poor in predicting OXA-48-like. We recommend CIM and eCIM for rapid identification of NDM-1 producers and mCIM at 4 ​h and MHT for detection of OXA-48-like. No one method can correctly detect both genotypes. As determined by ROC curves a zone of inhibition of 17 ​mm was considered adequate for detection of OXA-48-like and 16 ​mm of NDM-1 by mCIM at 4 ​h and CIM respectively.

اللغة الأصليةEnglish
الصفحات (من إلى)451-456
عدد الصفحات6
دوريةIndian Journal of Medical Microbiology
مستوى الصوت39
رقم الإصدار4
المعرِّفات الرقمية للأشياء
حالة النشرPublished - أكتوبر 1 2021

ASJC Scopus subject areas

  • ???subjectarea.asjc.2400.2401???
  • ???subjectarea.asjc.2700.2726???
  • ???subjectarea.asjc.2700.2725???
  • ???subjectarea.asjc.2400???
  • ???subjectarea.asjc.2700.2723???
  • ???subjectarea.asjc.2400.2404???
  • ???subjectarea.asjc.2400.2403???


أدرس بدقة موضوعات البحث “Reliability of carbapenem inactivation method (CIM) and modified carbapenem inactivation method (mCIM) for detection of OXA-48-like and NDM-1'. فهما يشكلان معًا بصمة فريدة.

قم بذكر هذا