TY - JOUR
T1 - Impact of splenectomy on circulating microparticles in patients with sickle cell anemia
AU - Boulassel, Mohamed Rachid
AU - Al-Rubkhi, Khoula
AU - Al-Qasabi, Jamal
AU - El-Ghamry, Islam
AU - Khan, Hammad
AU - Panjwani, Vinodh
AU - Qureshi, Rizwan Nabi
AU - Al-Mamari, Sahimah
AU - Al-Qarni, Zahra
AU - Al-Kindi, Mohamed
AU - Al-Nabhani, Abdulrahman
AU - Al-Gharibi, Khalaf
AU - Wali, Yasser
AU - Pathare, Anil
N1 - Publisher Copyright:
© 2023 John Wiley & Sons Ltd.
PY - 2023/9/3
Y1 - 2023/9/3
N2 - INTRODUCTION: Circulating microparticles (MP) are being described as potential biomarkers for disease activity in a variety of conditions including sickle cell anemia (SCA). However, relatively little is known about the influence of spleen status on MP levels in patients with SCA.METHODS: Using a prospective study design we characterize circulating MP in 144 patients with SCA in steady state by assessing their cellular origin and their relationships to spleen status defined by clinical and imaging findings. In addition, MP levels were studied according to demographic characteristics, clinical status, treatment modalities, and other hematological and biochemical parameters. Absolute plasma concentrations of MP were determined by flow cytometry.RESULTS: Patients with SCA displayed a 10-fold increase in levels of MP derived from red blood cell (RBC) and platelets (PLT) when compared to their healthy counterparts (p < 0.0001). Splenectomized patients with SCA have more pronounced levels of MPRBC and MPPLT, and remained elevated after several weeks of follow-up. Levels of MP were not significantly associated with spleen removal procedures, age, gender, clinical severity score, hydroxyurea therapy, hemoglobin F, and co-existence of glucose-6-phosphate dehydrogenase deficiency.CONCLUSION: Collectively, these results suggest that splenectomy affects circulating levels of MP regardless of the known SCA modifiers and correlates.
AB - INTRODUCTION: Circulating microparticles (MP) are being described as potential biomarkers for disease activity in a variety of conditions including sickle cell anemia (SCA). However, relatively little is known about the influence of spleen status on MP levels in patients with SCA.METHODS: Using a prospective study design we characterize circulating MP in 144 patients with SCA in steady state by assessing their cellular origin and their relationships to spleen status defined by clinical and imaging findings. In addition, MP levels were studied according to demographic characteristics, clinical status, treatment modalities, and other hematological and biochemical parameters. Absolute plasma concentrations of MP were determined by flow cytometry.RESULTS: Patients with SCA displayed a 10-fold increase in levels of MP derived from red blood cell (RBC) and platelets (PLT) when compared to their healthy counterparts (p < 0.0001). Splenectomized patients with SCA have more pronounced levels of MPRBC and MPPLT, and remained elevated after several weeks of follow-up. Levels of MP were not significantly associated with spleen removal procedures, age, gender, clinical severity score, hydroxyurea therapy, hemoglobin F, and co-existence of glucose-6-phosphate dehydrogenase deficiency.CONCLUSION: Collectively, these results suggest that splenectomy affects circulating levels of MP regardless of the known SCA modifiers and correlates.
KW - hemoglobin F
KW - hydroxyurea
KW - microparticles
KW - sickle cell anemia
KW - spleen
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U2 - 10.1111/ijlh.14162
DO - 10.1111/ijlh.14162
M3 - Article
C2 - 37661331
AN - SCOPUS:85169691623
SN - 1751-5521
VL - 46
SP - 141
EP - 147
JO - International Journal of Laboratory Hematology
JF - International Journal of Laboratory Hematology
IS - 1
ER -