Heterologous expression of chaetomium thermophilum Xylanase 11-A (CtX H-A) gene

Saiqa Wajid*, Shafiq Shahid, Farooq Latif, Zahid Mukhtar, Sher Afzal, Shahid Mansoor

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء


Chaetomium has a potential, source of xylanase and cellulase enzymes, both of which are required in the treatment of fibre in the poultry feed. The titre of the enzymes needs to be enhanced by using recombinant DNA technology for fulfilling the requirement of the industries. Efforts are made to construct prokaryotic and eukaryotic expression cassettes that can be cloned under specific strong promoters i.e., T7 and AOX1, respectively, and the enhancer elements to get the maximum, gene expression. In the present study BL2.1. E. coli and GS115 Pichia pastoris strains are used as model organisms to express the CtX 11-A gene in the presence of 1 mM IPTG and 100% methanol upto final, concentration of 0.5. In case of BL21 expression, the maximum xylanase activity was observed after 1.5 h in the presence of 1 % xylose, which was 2.302 U/ml and after 7 h in the presence of 0.5% lactose, was 1.708 U/ml. However, in Pichia pastoris the maximum production of xylanase was 2.904 and 0.006 U/ml as compared to control 0.484 and 0.06 U/ml, respectively.

اللغة الأصليةEnglish
الصفحات (من إلى)100-106
عدد الصفحات7
دوريةPakistan Journal of Scientific and Industrial Research
مستوى الصوت52
رقم الإصدار2
حالة النشرPublished - مارس 2009
منشور خارجيًانعم

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